The summer school aims to provide an overview of synthetic biology advances and their applications. Students will get hands-on experience in molecular cloning and genetic engineering. This course is designed for high school students and beginner level BSc students.
Laboratory work will focus on genetically modifying baker’s yeast (S. cerevisiae) with previously designed set of plasmid vectors. The constructed yeast strains will be used to study intricate mechanisms of regulation of gene expression and to illustrate the concept of logic gates in synthetic biology. Three distinct fluorescent proteins will be used as protein expression reporters. By combining different inputs to induce gene expression, the fluorescence of different reporter proteins will be measured as output.
Students will learn how to work in the molecular biology lab, lab safety rules, how to operate various laboratory equipment (e.g., PCR machines, centrifuges, vortexes, thermostats, etc.). They will learn the basic principles of logic gates and their applications in synthetic biology. Students will collect and analyse the data using different research methods, including live-cell flourescent microscope imaging, FACS, Western Blotting.
Ilona Faustova, Associate Professor in Molecular Biology
Format:
Summer course, hands-on workshop
Location:
Nooruse 1, Tartu
Course dates:
28 July - 8 August 2025
Language:
English
ECTS:
2
Study group:
High school students, beginner level BSc
Lecturers
Ilona Faustova
Artemi Maljavin
Juli Mukhadze
Vice Director of the Institute of Bioengineering, Associate Professor in Molecular Biology, Programme Director of Science & Technology and Bioengineering programmes
Junior Lecturer in Molecular Biology, Assistant of Programme Director
Junior Research Fellow in Molecular Biotechnology
Application deadline: 30 April 2025
Application period: 1 April - 30 April 2025 NB! All of the applicants are required to pay a non-refundable application fee of EUR 25. Your application will only be processed after the University has received the fee. The application fee is required to facilitate the admissions process and will not be refunded, regardless of the admission result.
Course fee: 600EUR
Includes: Study materials 8 days of academic work with lecturers Certificate of completion (2 ECTS) 5 cultural events in the evenings
NB! Transportation and accommodation costs are not included. The course fee does not cover participant's lunch during the summer school.
Before every practical assignment, instructors will explain the theoretical basis of the method. The students can take part in a cultural programme in the evenings.
Week 1
Sunday, 27 July
Arrival to Tartu
Monday, 28 July
Introduction to summer school: Schedule, goals, learning outcomes.
Introduction to safety rules and laboratory techniques
PCR amplification Estradiol Transcrition Factor
Lecture: How synthetic biology is changing the world
Restriction of PCR product and plasmid vector
Ligation of PCR product and plasmid vector
Tuesday, 29 July
Transformation of E. coli TURBO competent cells with Golden gate assemblies
Lecture: Logic gates and their applications in synthetic biology
Institute Tour
Wednesday, 30 July
Plasmid DNA extraction from transformed E. coli TURBO cells
Measuring DNA concentration
Restriction analysis of the extracted plasmid DNA
Preparing DNA samples for Sanger sequencing
Thursday, 31 Juli
In silico cloning in Benchling
Friday, 1 August
Analysis of Sanger Sequencing results
Yeast transformation
Lecture: The basics of homologous recombination and its application in synthetic biology
Saturday, 2 August
Free day
Sunday, 3 August
Free day
Week 2
Monday, 4 August
Check the S. cerevisiae colonies after transformation if the plasmid integrated correctly into yeast genome using PCR
Inoculate growth media with correctly constructed strains
Tuesday, 5 August
Induce different scenarios of protein expressions, collect samples for Western blotting
Prepare samples for western blotting
Wednesday, 6 August
Western blotting
Introduction of the Bioengineering MSc programme at the University of Tartu
Thursday, 7 August
Live-cell flourescent microscope imaging
FACS
Final discussion of the Results
Awarding of Diplomas
Friday, 8 August
Departure
The student completing the course will:
Know the main branches of synthetic biology
Will learn potential drawbacks and ethical issues raised by synthetic biology development
Know different molecular cloning techniques, including Golden Gate assembly and digest/ligate cloning
Be able to perform PCR, DNA gel electrophoresis, Bacterial and Yeast transformations, DNA extraction from bacterial cells.
Learn about different sequencing methods and how to analyze the results of Sanger sequencing
Learn the fundamentals of the Western blotting method and how the conduct western blot analysis.
Learn to analyse the live-cell flourescent microscope imaging data.
Learn the basics of FACS analysis.
In order to complete the course, the students must take part in all of the lectures and workshops and complete the given tasks on each day.
Entry requirements:
Online application form (application period April 1 - April 30 2025)
Motivation letter (up to 1 page) that demonstrates the applicant’s motivation to participate, his/her expectations about the programme, how participation in the summer programme relates to his/her studies and interests, and how the applicant plans to use the gained experience and knowledge in the future.
Transcript of academic records
Copy of passport
PS: Only complete applications including all annexes submitted by the deadline will be considered for selection.
Photo Gallery
Check out the photos from last year!
UniTartu Summer School Synthetic Biology and its Applications
Are you wondering how to get to Tartu and where to stay? Tartu is the second biggest city in Estonia and is, therefore, quite easily reachable. Tartu is famous for its sustainable transportation methods which make moving around the city very quick, cheap and convenient. In the City of Education there are many options of accommodation. You'll get to choose between a cheap stay at the dormitory with other international students or pick a fancier option in a hotel or apartment.
While the academic course is broaden your knowledge and learning skills, the cultural programme offers a range of fun and engaging cultural activities, introducing you to Estonia and the opportunity to interact and network with other participants in a lively environment. Students will have the opportunity to enjoy a range of day trips and events offered through the programmes and their experience in Tartu!
Students can choose a combined specialisation in either genetics and biotechnology; bioengineering and robotics; or chemistry and materials science. The general objective of the curriculum is to provide the graduates with a broad-based academic education in the natural sciences, which enables continuing studies on the Master’s level or commencing work in professions that require basic knowledge in the field and simpler working skills, such as at monitoring centres, different technological enterprises and laboratories as a member of a technologically competent lab personnel.
The main aim of the program is to educate specialists with skills and knowledge that will help them to find jobs and contribute to the success of various companies working on the field of industrial or medical biotechnology, circular bioeconomy, bioprocessing etc. The graduates will have understanding and practical experience covering the main industrial bioprocesses, fermentation, design of biological systems, industrial biotechnology, and biomedical technologies. The curriculum emphasises practical training, and provides internships in various companies that are active in the fields of biotechnology and biomedical technology.